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Question: 1 carefully consider the options and match the conditions of...

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1. Carefully consider the options and match the conditions (of plasmid and plate) with the expected growth.

+pGLO indicates transformation by the pGLO plasmid
-pGLO indicates no plasmid

-pGLO on LB/Amp?

-pGLO on LB/Ara?

+pGLO on LB/Ara?

Possible answers include:

a. No growth at all

b. Growth of both white color and green fluorescent colonies

c. Growth of only green fluorescent color colonies

d. Growth of only white color colonies

2. What genes are required in the pGLO plasmid for production of fluorescent bacteria on LB/Ara plates?

There may be more than one correct answer. Identify all for credit.

a. None of these genes

b. bla

c. araC

d. All of these genes

e. GFP

3. What genes are required in the pGLO plasmid for growth of e. coli bacteria on nutrient broth plates?

There may be more than one correct answer. Identify all for credit.

a. None of these genes

b. bla

c. araC

d. All of these genes

e. GFP

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