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Question: 1 give a 6bp palindrome that could function as a...

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1. Give a 6bp palindrome that could function as a restriction enzyme recognition sequence. Give the sequence on both strands, indicate polarity, and identify the axis of symmetry with a dotted line. 2. n is the number of cuts that a restriction enzyme makes in a chromosome. For a circular chromosome, the number of fragments with digestion created-n. Draw a simple sketch below that demonstrates this relationship. 3. How would you represent the number of fragments created for a linear chromosome (hint, use n)? 4. Draw a simple sketch below that demonstrates the relationship in 3. 5. Mspl and Hpall restriction sites and their positions in bp along a sequence of interest are shown in the map below. Hpall is methylation sensitive, while Mspl is not. That is, if the DNA that comprises an Hpall restriction sequence is tagged with methyl groups, the sequence will not be cut by Hpall. 200 1800 0 100 1000 1500 2000 bp Mspl Mspl Hpa Mspl Hpall What are the sizes of fragments that you would expect from digesting this DNA simultaneously with Mspl and Hpall if: a) the DNA was not methylated, and b) the DNA was methylated?

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