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2 carefully place the microscope on the table 3 identify...

Question: 2 carefully place the microscope on the table 3 identify...

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2. Carefully place the microscope on the table. 3. Identify the head, the arm, and the base of the microscope.Using the Microscope to View 4. Identify the ocular lens(es). Activity 2 Objects Is your microscope a monocular microscope or abin- Learning Outcomes ocular microscope? Ocular lenses typically magnify objects 3. Identify the rotating nosepiece that connects the objec. 1. Demonstrate the proper use of the microscope to view 2. Calculate total magriication for each objective lens 3. Determine the working distance for each objective lens. Materials Needed rimes. tive lenses to the head of the microscope. Rotate the lenses can be locked into place. Each objective lens is Compound light microscope labeled with its magnifying power O Letter e microscope slide Note the magnifying power of each objective lens andO Immersion oil and dropper record it in the following list. Scanning objective Low-power objective: O Lens paper O Lens-clearing fluid Instructions High-power objective: Oil-imumersion objective: 1. Obtain a letter e slide. Examine the orientation of the e before you place the slide on the stage. Sketch the appearance of the lettere in the following rectangle. Total magnification is calculated by multiplying the power of the ocular lens by the power of the objective lens. For example, the total magnification of the scan- ning objective is 10x (the power of the ocular lens) X 4X (the power of the scanning objective) 40X 6. Identify the stage, the platform on which the slide sits. Note the hole in the stage through which light passes. What is the name of this opening? Is the e right-side up or upside down? Is the e oriented left to right or right to left? Does your microscope have a mechanical stage, as shown in Figure 4-2, or does it have spring clips to hold the slide in place? 2. Plug in your microscope and turn it on. 3. Rotate the scanning objective into position, and place the slide on the stage. Record the magnification of the scanning objective in the microscope summary table on the next page. Calculate the total magnification 7. If your microscope has a mechanical stage, identify the two adjustable mechanical stage knobs that control the movement of the slide. when urincg the scanniii he summary chart. Using the stage adjustment knobs, move the slide around until the letter e comes into the field 4. 8. Identify the larger coarse adjustment knob and the smaller fine adjustment knob located on the arm just above the base of the microscope. Rotate the coarse adjustment knob to move the stage up and down. This 5. Bring the letter e into focus using the coarse adjust knob allows for gross focusing of the specimen, whereas the fine adjustment knob allows for fine focusing ment knob. Once it is mostly in focus, use the fine adjustment knob to bring it into sharp focus. Sketch appearance of the letter e in the circle representing the field of view in the summary chart. 9. Identify the condenser, which concentrates light on the specimen. The iris diaphragm lever regulates the amount of light passing through the condenser. The passage of more light through the condenser decreases contrast between structures; the passage of less light through the condenser increases contrast between structures. Is the e right-side up or upside down? Is the e oriented left to right or right to left? Using the mechanical stage control knobs, move t 6. In which direction does the image of the letter e move as you look through the ocular lens(es)? 10. Identify the substage light located in the base. The light slide away from you control knob, often a rheostat dial, controls the bright- ness of the light. UNIT 4 | The Microscope

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