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Question: 3 a list factors that affect movement of dna in...

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3. A. List factors that affect movement of DNA in an agarose gel electrophoresis B. You were given to study a linear DNA sample by agarose gel electrophoresis. You were also given a standard DNA size maker. After electrophoresis and staining of DNA you found several bands in standard DNA size marker (M) and one band in sample (S) DNA. Size of each standard DNA molecule and their distance from the origin of electrophoresis was provided (see picture below. i) Draw a standard curve using the graph paper provided. Make appropriate labeling axis. in X and Y ii) Determine size (kb) of DNA
4. A. What is a restriction endonuclease? B. You were given a plasmid DNA( sequence and asked to determine the number of gel electrophoresi aircular) and viral (linear) DNA of unknown nucleotide EcoR1 site(s). Ater EcoR/digestion, agarose s and DNA staining of the gel following results were obtained. M Plasmid Virus Origin Direction How many EcoRI sites were present in the plasmid DNA? How many EcoRI sites were present in the viral DNA?
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