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Question: 418 substrate requirements for continuous culture...

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4.18 Substrate requirements for continuous culture

4.18 Substrate requirements for continuous culture The acetic acid bacterium Acetobacter pasteurianus is cultured under aerobic conditions in a continuous fermenter using ethanol (C2H,O) as the substrate and ammonia as the nitrogen source. Under these conditions, acetate accumulation is completely suppressed so that biomass is the only major product. The rate of ethanol consumption is 150 gh1 and the rate of biomass production is 45 gh (a) What is the rate of oxygen consumption during the culture? (b) Ammonia is fed to the culture at a rate of 20 g h 1. At what rate does unreacted ammonia leave the reactor?

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