Question: a hypothetical scenario is that your forward primer is engineered...
A hypothetical scenario is that your forward primer is engineered with BamH1 and the reverse primer is engineered with HindIII. Using these restriction enzymes for cloning the PCR product into the pGEM vector, would this work. (Note: Plac is a promoter that can be used to drive the expression of an inserted gene).
Could someone please guide me through the steps of how to solve this PCR problem? I'm at a loss as to where to start