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Question: a number of factors influence the melting properties of a...

Question details

A number of factors influence the melting properties of a linear, double-stranded DNA molecule in a 0.25M sodium chloride solution. Considering this, explain each of the following observations:

(a) The Tm increases in proportion to the length of the molecule

(b) As the concentration of sodium chloride is decreased, the Tm decreases

(c) Renaturation of single strands to form double strands occurs more rapidly when the DNA concentration is increased

(d) The Tm value is reduced when urea is added to the solution

(e) The Tm value is reduced when cytosine bases undergo deamination

(f) When one of the strands of the DNA is replaced by the same sequence of RNA, the Tm of this DNA:RNA hybrid double helix is higher than the Tm of the DNA double helix

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