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Question: a rat liver cytosol preparation is incubated with acetate and...

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A rat liver cytosol preparation is incubated with acetate and all cofactors necessary for the biosynthesis of palmitatee. Which C atoms of palmitate will be radioactive when preparation contains: a) H14CO32- b)14CH3COO- (C is not the more abundant and very stable C-12 but instead it is C-14) 14CH3COO- means that only the methyl carbon contains the C-14 isotope. What do the cells do with the labelled acetate? 14CH3COO- + ATP --> acetyl-CoA + AMP + PPi The enzyme that catalyzes the above reaction is acetyl-CoA synthetase I. Support your answer with the relevant chemical reactions. Cannot just show the labelled C!

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