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Question: a researcher is interested in following telomere localization in yeast...

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A researcher is interested in following telomere localization in yeast. Although we know they are attached to the nuclear membrane, she has reason to believe that telomeres are more dynamic than initially thought. Which of the following strategies would work best to test this hypothesis?
Create a monoclonal antibody against a telomere resident protein and perform immunofluorescence using differential interference contrast (DIC) microscopy.
Create a monoclonal antibody against a telomere resident protein and perform immunofluorescence using confocal microscopy.
Create a monoclonal antibody against a telomere resident protein and perform immunofluorescence using total internal reflection fluorescence (TIRF) microscopy.
Tag a telomere resident protein with GFP and perform Fӧrster resonance energy transfer (FRET).
Tag a telomere resident protein with GFP and perform fluorescence recovery after photobleaching (FRAP).
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