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Question: after pcr both the vector pet28a and the result of...

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After PCR, both the vector pET-28a and the result of the PCR are digested in Xhol and Ndel. Following this, the two are combined with the addition of the enzyme Ligase. Question 7 What is the role of the Ligase? Type your response here

In order to introduce your new DNA into E. coli, the process of heat shocking a cell is used. This process involves heating cells to around 42-C before cooling them in ice. Question 10 What is the point of using heat in this transformation? Type your response here 40 E80

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