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Question: billy is asked to freeze his cells from a t25...

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Billy is asked to freeze his cells from a T25 flask at the concentration of 106 cells/ml. After trypsinizing his cells with 1ml trypsin, he adds 4 ml of media to quench the trypsin. Using a hemocytometer, he counts 315 cells in ten squares. How much freezing media does he need to freeze his cells in and how is he going to freeze his cells?

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