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  3. c to insert your pcr product into the plasmid shown...

Question: c to insert your pcr product into the plasmid shown...

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c. To insert your PCR product into the plasmid (shown, with positions of restriction sites indicated, on the right), you add an EcoRl site to Primer 1 and an Xbal site to Primer 2, and after the PCR reaction, you cut both PCR product and plasmid with EcoRl and Xbal and ligate plasmid and insert together using DNA ligase. After transforming the ligated plasmid into E. coli, you isolate it and cut it with EcoRl and BamHl. Supposing the PCR product is 2,000 bp, list the size(s) of the DNA fragment(s) you expect if your cloning was successful? BamHI Xbal EcoRI (100) (200 BamHl (400) Plasmid (4,000 bp)

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