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Question: chapter 2 study guide 1 place the following in order...

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Chapter 2 Study Guide 1. Place the following in order from largest to smallest red blood cell atom, paramecium, polio virus, E. coli bacterium, and globular protein. Which can be seen with the light microscope? With the electron microscope? What is the approximate diameter of a typical bacterial cell? Why are eukaryotic cells typically larger than prokaryotic cells? 2. 3. The objective lens of a compound microscope magnifies the specimen 10, 40, or 100 fold. What does the ocular lens magnify? What is the total magnification if the ocular lens is 10x and the 100x objective is in place? 4. For the electron microscope, what structure is analogous to the lens in the light For what purpose would you use each type of microscope: a) bright field, b) dark field; c) phase contrast; d) fluorescence; e) TEM; f SEM? 5. For what purpose would you use each of the following a) simple stain; b) negative stain; c) acid-fast stain; d) Gram stain? 6. 7. Briefly describe how each type of microscope and each staining procedure from questions 5 and 6 work 8. List the steps of the Gram stain procedure. What color would a Gram negative organism appear after each step? a. b. Is the Gram stain used to help identify bacteria, viruses, fungi, or protozoa? Why is it important to know the Gram reaction of this type of pathogen? How might the Gram stain result impact treatment options in a clinical setting?
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