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Question: draw the first 3 cycles of pcr indicating the intermediate...

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Draw the first 3 cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5' to 3'), and the size of the desired target double stranded DNA product.

A thorough understanding of the figure belo each letter. The primers are indicated by the arrows for both the sense and anti-sense DNA strands. In the following questions, a copy of DNA refers to a double-stranded piece of DNA. process of PCR will help in the analysis of your DNA gel. The w represents a segment of double-stranded DNA with 100 base pair segments denoted by 5 A B C D E F G H A. Draw the first three cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5- 3), and the size of the desired target double-stranded DNA product. Include the temperatures and their significance for each step in the PCR reaction for the first cycle. (9 points, 3 pts per cycle) For each cycle, indicate how many copies of target double stranded-DNA and intermediate DNA (the DNA includes target DNA region plus a bit of the flanking DNA and/or the original DNA strand) you would have after each cycle assuming you began with one double strand DNA template. (6 points, 2 pts per cycle) B.

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