Question: each of the following bottles contains a 200 ml of...
Each of the following bottles contains:
a. 200 ml of NaCl at a concentration of 5 M
b. 200 ml of Tris at a concentration of 1 M, and
c. 1 L of distilled water
Calculate the volume in millilitres (ml) needed to draw from each of the bottle above to prepare 1 litre (L) of buffer solution that contains 150 mM of NaCl and 50 mM of Tris. (5 marks)