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Question: each of the following bottles contains a 200 ml of...

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Each of the following bottles contains:

a. 200 ml of NaCl at a concentration of 5 M

b. 200 ml of Tris at a concentration of 1 M, and

c. 1 L of distilled water

Calculate the volume in millilitres (ml) needed to draw from each of the bottle above to prepare 1 litre (L) of buffer solution that contains 150 mM of NaCl and 50 mM of Tris. (5 marks)

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