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Question: enzyme and enzyme kinetics chapter 3 concepts 1 standard michaelismenten...

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Enzyme and Enzyme Kinetics (Chapter 3) Concepts: #1: Standard Michaelis-Menten Kinetics Consider an enzyme that follows standard Michaelis-Menten kinetics and has the following kinetic constants:

KM=1.2x 10-3 M k2=6.0 x 10s Eo=1x10-4 M

a. What is the value of the maximum rate VM?

b. Prepare a hand-drawn quantitative plot on graph paper (not a simple sketch nor an EXCEL-generated graph) of the enzymatic reaction rate versus substrate concentration (like Fig 3-3) using the kinetic parameters given above. Be sure to label the axes and include numeric values on the axes.

c. Based upon your hand drawn saturation plot, at what substrate concentration is the enzymatic reaction rate 75% of VM?

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