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Question: enzymes lab report enzyme structure and function define the following...

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Enzymes Lab Report

  1. Enzyme Structure and Function

  1. Define the following:

  1. enzyme
  1. substrate
  1. Explain how enzymes function.

  1. Go to Lab, Section I, Exercise 1 to participate in an activity that demonstrates the importance of enzymes. Record the color intensity of the solution in each test tube at five and ten minutes.

Time

(min)

Tube S1

Potato Extract + Catechol

Tube S2

Potato Extract + Water

Tube S3

Catechol + Water

0

5

10

  1. How can benzoquinone be detected?   
  1. Why were potatoes used in the experiment?

  1. Enzyme Specificity

  1. Based on Section II, Exercise 2, as determined by color intensity, to what extent does catechol oxidase react with hydroquinone?
  1. What was the role of hydroquinone in the experiment?

  1. Temperature and Enzyme Activity

  1. Based on Lab, Section III, Exercise 3 record the relative color intensity at five-minute intervals to determine the temperature range for catechol oxidase.

Time

(min)

Tube 1

4oC

Tube 2

23oC

Tube 3

40oC

Tube 4

60oC

Tube 5

80oC

Tube 6

100oC

0

5

10

  1. Construct a bar graph of the data for the ten-minute readings. Label axes and provide units. Sign, date and prepare an image of your graph and include it with this lab report.

  1. What is the optimal temperature range for catechol oxidase activity?   

  1. pH and Enzyme Activity
  1. Within what pH range will catechol oxidase catalyze catechol and oxygen to form benzoquinone?   
  1. Define pH.
  1. What is the role of catechol in the experiment?
  1. Which test tube contains the most acidic buffer solution?
  1. Cofactors and Enzyme Activity

  1. What is a cofactor?

  1. What cofactor is necessary for catechol oxidase activity?

Summary Questions

  1. What was the role of catechol oxidase in the experiments?   

  1. What was the purpose of developing a color intensity scale?

  1. Identify three factors that influence the rate of enzymatic reactions.
  1. Examine the data in Exercise 3 and suggest a hypothesis explaining why people with a fever feel tired and listless.   

  1. People who have been suddenly submerged in cold water and remained for long periods of time have survived. Examine the data in Exercise 3 and explain how this is possible.   

  1. How does pH range affect the function and structure of catechol oxidase?   

  1. The stomach maintains a pH of approximately 3 to 5. Do all enzymes function at this pH level? Examine the data in Exercise 4 and determine if catechol oxidase would function in the stomach.   

  1. What is enzyme specificity? Give an example.   

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