Question: for your experiment you need 1 x 105 cellsml after...
For your experiment, you need 1 x 105 cells/ml. After collecting the buffy coat from human blood and performing a 1:100 dilution and adding 100 µl of the diluted buffy coat to 100 µl of trypan blue, you see the following results in your hemocytometer.
Hemocytometer=86 cells in 4 quadrants
A) What is the concentration of cells in the buffy coat? Show your work.
B) What dilution do you need to make of the buffy coat to achieve the desired concentration of 1 x 105 cells/ml? Show your work.
C) What volume of the original buffy coat do you need to achieve the desired concentration in 10 ml of HBSS + 5% FCS? Show your work.
Please answer part (C) and explain