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Questions 1. What is the function of immersion oil? What is the refractive index of air? 2. Did you see unstained areas in the Bacillus? What are they? 3. Why is the 40X objective not used when looking at a stained preparation? 4. Explain the function of the condenser and the iris diaphragm. 5. Why do you initially focus on the wax pencil mark instead of the stain? 6. What is the minimum magnification needed to observe most bacteria? 7. Explain the names streptococcus, staphylococcus, bacillus, and micrococcus. 8. Where do you discard used slides?
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