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Question: heres the lab heres the lab setup...

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From the dilution series you set up today: 1) What is the dilution factor of dye in the 40 tube of the 1:10 dilution series (dye was undiluted or neat in well 1) 2) What is the concentration of dye in 7th tube of the 1:2 series? (dye was 10 mg/ml in well 1)

Here’s the lab:

B) Set up dilution series for the standard and for your unknown, 1) Make a 1:10 dilution series and at least two 1:2 dilation series of the standard in a 96 well plate A 1:10 dilution consists of 1 part solute (whatever you are diluting) and 9 parts diluent (whatever you are diluting in) for a total of İO parts. The solute in a io dilution is in o concentrated as when it started. Mtpl iorレ A 1:2 dilution involves mixing equal volumes of 2 solutions and the solute concentration is cut in half. You will all be using the same initial neat solution (10mg/ml). It is too concentrated for the plate reader to read so you will need to dilute it to a working concentration. We are using water as a diluent. Leave well Al as a blank. Fill with diluent only. ea uu 1st add the appropriate volume of diluent to all tubes that will need it. 2nd add some neat (undiluted) solution to the first tube in each series. that will need it, 3rd transfer some volume of neat to the second tube (the first tube with diluent), mix, then transfer the same volume from that tube into the next, etc. etc etc. C1 You can use whatever volume youd like, up to 200 microliters, but all wells in a series must,m have the same final volume of at least 100 microliters. Dilute the neat (undiluted) standard solution until all visible color is gone. This will require 1 row (A) of a 96 well plate for the 1:10 and 2 rows for each 1:2 series. You should be able to use one tip to do all transfers. It is acceptable to move a tip from a very dilute solution to a concentrated one, but NOT vice versa. ASK if this doesnt make sense! 2) Make a 1:2 series of your unknown so at least one well will fall in your standard curve from above. (You dont know where this will be yet, so make sure you dilute the sample until all color is gone.) 3) Read plate on ELISA plate reader 4) Use the averages of your data from the linear regions of your 1:2 series to plot a standard curve using Excel to determine the initial concentration of unknown in your tube.

Here’s the lab setup:

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