1. Science
  2. Biology
  3. hexokinase is an important ename of glucose metabolism we will...

Question: hexokinase is an important ename of glucose metabolism we will...

Question details

Hexokinase is an important ename of glucose metabolism we will encounter later in the semester. If comes in several isoforms, different versions of the proteins with slightly different properties, but they essentially all catalyze the same the reaction, the ATP dependent phosphorylation of glucose. glucose + ATPglucose- 6-phosphate + ADP reticlooge in the following experiments, the authors performed an analytica separation of protein from immature red blood cells blood cells by ion exchange fractions for hexokinase activity and compared the results with samples from brain and skeletal muscle red blood cells brotn etal musele FRACTION NUMPR Fro. 1. DEAE-cellulose chromatography of hexokinase ac- tivities from reticulocytes (A), total red blood cells (B), brain (C), and skeletal muscle (D) of rabbit. The columns were devel- oped with 280 ml of a linear gradient of KCi from 0 to 04 M in &mN potassum phosphate buffer, pH 75, containing 9% (v/v) glycerol and operated at 30 ml/h. Fraction of 25 ml were collected. hexokinase activitiesKCl gradient.
okinase in brain vs. skeletal muscle of rabbit? How doest can you say about hex to red blood cells as they mature? ll you about 2. The proteins are separated on a diethylaminoethyl resin at pH7 5, what does this the isoforms of hexokinase in skeletal muscle. CH-CH CHİCH-N-H CH-CH3 Cellulose 3. In order to purity the enzyme further, the authors performed a large scale preparation of hexokinase separated from other proteins by DEAE-cellulose column chromatography after ammonium sulfate precipitation. Looking at the graph below, is this a good purification step? Justify your answer
Pooled 0.60 1.5 0.45 : 10 a.1 10 20 30 4050 0 FRACTION NUMBER FrG. 2. DEAE-cellulose column chromatography of the 35 to 65% ammonium sulfate fraction. The sample was applied to the column (1 x 20 cm) in 5 mM sodium potassiurm phosphate buffer, pH 7.5, containing 9% (v/v) glycerol, 5 mM glucose and operated at 30 ml/h. The hexokinase activities was eluted with a linear gradient (140 ml in each chamber) of KCl from 0 to 0.4 M in the same sodium potassium phosphate buffer, pH 7.5, and 2.5-ml fractions were col- lected. ● 0, absorbance at 280 nm;--, KCl gradient; O O hexokinase activities. Tubes 32 to 40 and 45 to 57 were pooled for further purification.
Solution by an expert tutor
Blurred Solution
This question has been solved
Subscribe to see this solution