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Question: how to make 1x biotinylated tsh detection antibody...

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How to make 1x biotinylated TSH detection antibody?
9.3 1X Biotinylated TSH Detection Antibody Briefly spin the Biotinylated anti-Human TSH vial before use. Add 100 HL of 1X Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can either be stored at 4°C for 5 days or aliquoted and frozen at -20°C for 2 months). The detection antibody concentrate must be diluted 80-fold with 1X Assay Diluent B prior to use in the Assay Procedure.
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