1. Science
  2. Biology
  3. i am having trouble figuring out the dilution factors assuming...

Question: i am having trouble figuring out the dilution factors assuming...

Question details

I am having trouble figuring out the dilution factors. Assuming CFU/mL = [# of colonies * (1/dilution factor)] / amount plated in mLEXPERIMENT NO. 1 - SELECTED QUESTIONS 1. You have performed the indicated series of dilutions. For cach, give the titer of the original culture (show your calculations). A. Tube → 10 μし→990 μL PBS 300 μ 700 μL PBS 200 μし→ 800 μL LB broth 100 μ L→ LB agar plate Colonics on plate after 24 hr 2 Titer of original culture- cfu/ml. B. Tube → 100 uL → 900 μしH2O 1 μし→9999 μL LB broth 1 uL →49 μしLB broth 50 μL→ 950 uL LB broth 100 uL→ LB agar plate Colonies on plate after 24 hr 130 cfu/ml. Titer of original culture = C. Tube → 10 μし→490 μL PBS 100 μし→ 1900 uL PBS 500 μ 500 μL PBS 100 ul→ LB agar plate Colonies on plate after 24 hr 353 Titer of original culture- cfu/ml.

Solution by an expert tutor
Blurred Solution
This question has been solved
Subscribe to see this solution