Question: in biochemical assays ethylenediaminetetraacetic acid commonly known as edta c10h16n2o8...
In biochemical assays, ethylenediaminetetraacetic acid, commonly known as EDTA (C10H16N2O8) is an extensively used chemical in the preparation of buffers and solutions.
a. Calculate the amount (weight) needed in grams (g) to prepare 500 ml of solution containing 5 mM EDTA. (2 marks)
b. By adding the entire solution prepared in (a) to 500 ml of a second buffer solution containing 300 mM of NaCl and 5 mM EDTA, calculate the concentration of NaCl and EDTA in the new solution mixture. (3 marks)