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Question: is the cohesin protein rec8 involved in sister chromatid cohesion...

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DNA REC8 Merge Figure 2 A and B) Chromosome-associated REC8 was detected by immunocytochemistry in young and old oocytes at ML Representative images (A show DNA (Sytax, green) and REC8 (red); scale bar reprosents 5 am. Similar rosults wore ob- tained with multiple young (n 11) and old (n 9) oocytes, and the REC8 fluorescence intensity was quantified (B). (C and D) Total REC8 levels in young and old oocytes at Mi in-80 oocytos in each lane) were determined by westerm blot with TUBB as a loading control. A representative blot is shownc similar results were obtained in two independent experiments, and band intensities were quanti- fied (D). Shown are results from one experiment (E) Levels of chromosome-associated REC8 were mea sured as in (A) with oocytes from mice at different ages in months): 3 (n-21),6 n-21) 9 (n-19), 12(n-21),15 (n 7), 17 (n 3). (F and G) Mil eggs from mice at various ages D 250 1000 Young Old REC890 kDa 600 150 O REC8 were analyzed to determine interkinetochore TUBB distances (F) as in Figures 1A-1C, and the por TUBB 50 kDa 100 centage of eggs with chromosome segregation orrors, including anouploidios and soparatod sister chromatids, was determined (G). Mice were used at the following different ages in months):3(n 39), 6 (n > 28),9 (n-20, 12 (n > 24), 15 (n>14). 17 in -16 See also Figure S2 For (B). (E), and (F), the data are expressed as mean standard error of the mean Young Old Young Ok E 10001 045 0.40 035 0.30 600 0.20 3 6 9 12 15 17 Age (months) 3 6 9 12 15 17 Age (months) 3 6 9 12 15 17 Age (months)Is the cohesin protein REC8 involved in sister chromatid cohesion and aneuploidy in young versus old oocytes? If so, what are the connections among REC8, sister chromatid cohesion, and aneuploidy in young versus old oocytes?

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