Question: knowing whether a hemoglobin is in tstate vs rstate cannot...
Knowing whether a Hemoglobin is in T-state vs. R-state cannot be determined by simply looking at relative sizes of the cavity, since this will appear differently depending on what angle of the pymol structure you are using as your baseline cavity measurement. We learned in class that ionic bonds or salt bridges stabilize the T-state. You are asked to take measurements of the potential interaction between two groups that can form an ionic bond (since you have a full positive on R and a full negative on D). Depending on the measurements you obtain, you would have to deduce whether that distance can support (or not support) an ionic interaction. This would then deduce information on T vs. R state.
My question is how would you know if a particular distance could support the ionic interaction?
(The problem im doing the distances are 24.6 and 21.6)