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Question: lab 1 measurement pipette use and the standard curve experiment...

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LAB 1: Measurement, Pipette Use, and the Standard Curve Experiment 2 Standard Curve Table 1 0.15M NaCl for2.0 mL final volume Tube Final Protein Concentration BSA protein stock Bradford 0.5 mg/mL) needed Reagent 1.8 mL 1.8 ml 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 50 μg/mL 25 ug/mL 10 μg/mL 7.5 μg/mL 2.5 μg/ml ML 0 μg/mL
Concentration Calculations and Dilutions Page 9 of 13 When calculating concentrations, it is very important to pay attention to the units contained on each number (ie 10mg/ml vs 1M vs 1mo/L). Starting with a stock solution with a high concentration you will need to be able to calculate how much solvent and solute to mix together to create a solution at a new, lower concentration. One equation that can be very helpful in calculating this is C1V1-C2V2. Lets work through a practice problem: You have a 1mg/ml stock solution and wish to dilute it to 1ml of 0.1mg/ml solution. How much of the stock solution do you add? C1V1 C2V2 (Concentration 1s stock solution; V1 the Volume of stock solution you need to achieve Concentration 2 in Volume 2) 1mg/ml x V1 -0.1mg/ml x 1ml (solve for V1) 1 mg/ml x V1 . 0.1 mg ← Note that the mi units cancel each other out when we multiply them. V1- 0.1ml Note that the mg units cancel each other when we divide them. By treating the units as fractions and using basic algebra with them we can easily calculate that by diluting 0.1ml of our stock solution into 0.9ml of solvent we will make a 0.1mg/ml solution. By using these basic rules, you can calculate the dilution rate of any value.
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