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Question: lecture activity 2 original culture 01 m 2 ml 99...

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Lecture activity 2 Original culture 0.1 m 2 ml 9.9 ml 4 ml 18 ml 5 ml 9 ml What are the individual and total dilution factors for each tube (A 0.1 through E)? (4 pts) 0.1 ml28 295 Colonies per plate What is the CFU per ml of the 26 310 original starting culture? (2 pt) 290 Using 10-fold dilutions, design and draw out a dilution scheme for counting CFU per ml with a starting culture of ~ 3 x 108 CFU per ml. Your scheme should include plating 0.1 ml from three different dilutions with the middle dilution producing a countable plate (ex. you would platee dilutions 3, 4, and 5 with plate 4 expected to contain countable colonies) List IDF and TDF for every dilution in your series, and estimate how many colonies you would expect to grow at each dilution. (4 pts)

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