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Question: list the types of substances to which each of the...

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List the types of substances to which each of the following chromatographic methods is most applicable: (a) Gas-liquid (b) Liquid adsorption (c) Liquid-liquid partition (d) Reversed-phase partition (e) Ion exchange (t) Gel permeation (g) Gas-solid (h) Gel filtration 0) Ion-pair 2. 3. Two components in an HPLC separation have retention times that differ by 15 s. The first peak elutes in 9.0 min and the peak widths are approximately equal. The dead time t was 65 s. Use a spreadsheet to find the minimal number of theoretical plates needed to achieve the following resolution R, values: 0.50, 0.75,0.90, 1.0, 1.10, 1.25, 1.50, 1.75, 2.0, 2.5. How the results change if peak 2 were twice as broad as peak 1? 4. The following data were obtained by gas-liquid chromatography on a 40-cm packed column: Compound1.9 tR, min W. min Air Methylcyclohexane 10.0 Methylcyclohexene 10.9 Toluene 0.76 0.82 1.06 13.4 Calculate (a) An average number of plates from the data (b) The standard deviation for the average in (a) (e) An average plate height for the column (a) The retention factor of each compound (b) The distribution constant for each compound (c) The selectivity factor for methylcyclohexane and methylcyclohexene

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