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Question: methotrexate works as an anticancer drug by competitively inhibiting the...

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Methotrexate works as an anticancer drug by competitively inhibiting the enzyme dihydrofolate reductase. A set of experiments was done using 10 nM dihydrofolate reductase, a range of dihydrofolate concentrations (1μM – 500 μM) and 0, 5 nM and 20 nM methotrexate. The initial rates of each of these reactions were measured.

Which of the following figures shows the most likely outcome of these experiments? (Please be sure to discuss why you choose your answer and why the other choices are incorrect).

te A. 5 nM methotrexate 5 nM methotrexate 20 nM 0 methotrexate methotrexate /[dihydrofolate] l/[dihydrofolate] C. 500 M dihydrofolate ljuM dihydrofolate 50 M dihydrofolate dihydrofolate 500 pM dihydrofolate l/[methotrexate] I/[methotrexate]

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