Question: miniprep and restriction analysis of plasmid dna how does the...
Miniprep and restriction analysis of plasmid DNA:
How does the restriction enzyme digestion of the plasmid inform whether the gene is inserted in the correct orientation? Can you tell by the number of cuts (bands) seen in the gel run afterwards? Directional = 1 band. Non-directional = 2 bands? Or something along those lines?