1. Science
  2. Biology
  3. need help verifying these answers if any are wrong can...

Question: need help verifying these answers if any are wrong can...

Question details

In the process of intrinsic transcriptional termination, what RNA structural features are important to the process? A A specific sequence of bases at the 3 end of an mRNA is able to catalyze its own excision, thereby terminating transcription o B. A stem loop that forms because of complementarity between bases making up two inverted repeat sequences in the termination sequence. The C mal domain of RNA pol Il serves as a scaffold upon which the cleavage and polyadenylation complex resides, therefore enabling efficient transcription termination. OD. The rut sequence in the mRNA forms a structure that binds Rho protein, which causes mRNA release and transcription termination. QUESTION 12 Imagine that you do this experiment: you create a synthetic gene, based on what you know about the insulin gene in humans. You create a strain of E. coli in which this synthetic gene has been inserted into the bacterial chromosome. After checking for expression of your synthetic gene, you discover that no mRNA is produced. Which of the following is a possible explanation for this observation? O A TFiD and the other general transcription factors are not ableto recognize a prokaryotic promoter, therefore they cannot assemble on the promoter of the synthetic gene in a bacterial cell OB. The synthetic gene has a prokaryotic promoter that is missing the TATA box or GC box tus o sigma subunit can bind and recruit RNA polymerase. OC.The synthetic gene has a eukaryotic promoter that will not be recognized by any of the E. colli sigma-70 subunits, thus RNA polymerase will not bind to the promoter D The gene is lacking the Shine-Delgarno sequence, therefore RNA polymerase wont be able to assemble on the promoter with any of the sigma subunits QUESTION 13 You are studying the expression of a gene in a certain type of eukaryotic cell. Under normal conditions, you measure that the gene is expressed at a certain value. You are able to experimentlly alter a nucleotide pair at position -75 relative to the transcription start site. In the altered cells, you now measure a reduced rate of gene expression. 50% the value that you measured from normal cells. What is a reasonable interpretation or hypothesis to explain this observation? O A You likely altered a base in a sequence that RNA polymerase II requires for binding, therefore i is less able to recruit general transcription factors to the promoter to assemble the pre-initiation complex. . The mutation likely created an improved binding site for TFIID and the other general transcription factors, thus they are less likely to disassemble to form a new pre-initiation complex. С You kely altered a base located in a consensus sequence of the promote and the general transcr tion factors have a lower a nity or the mutated sequence therefore transcription initiation complexes form at a roduced rate D The mutation most likely altered a consensus sequence for sigma subunit binding, therefore the appropriate sigma subunit is less likely to bind to the sequence in the promoter to recruit RNA poll. Need help verifying these answers. If any are wrong can you explain why and what the correct answer is? Thank you.

Solution by an expert tutor
Blurred Solution
This question has been solved
Subscribe to see this solution