1. Engineering
  2. Chemical Engineering
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8. To make up a solution of phosphate buffered saline (PBS), you need 5 mM Na2HPO4 (anhydrous: FW- 141.96), 0.15 M NaCl (FW = 58.44) and 1 mM KH2PO. (FW you need to make up 1 liter of PBS? 136.09). How many grams of each will 9. You want to add PMA to 25 mL of HL-60 cells so that you have a concentration of 2 nM, Your stock solution is 1 며M. What is the volume of stock solution needed? 10. The molecular weight of vincristine, a pharmaceutical drug, is 923.04. It is supplied as 1 mg of powder per vial. You want to treat your culture cells with a final concentration of 5 pM, vincristine and will need 20 mL of cells for your experiment. You want to add 1 μ L of solution. What concentration does your stock solution need to be?

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