1. Science
  2. Biology
  3. please answer only if you have all 10 answers please...

Question: please answer only if you have all 10 answers please...

Question details
Please answer only if you have all 10 answers, please give all correct answers for biochem . Thank you
Use the information gathered in the CRISPR Simulation to answer the following question. Which of the following enzymatic activities is not required as part of the CRISPRICas9 system? O ligase O endonuclease O polymerase O exonuclease O All of the listed enzymatic acitivies are required.
Use the information gathered in the CRISPR Simulation to answer the following question. CRISPRICas9 system can be considered a bacterial adaptive immune system because O It stores molecular elements of infectious agents to fight off future infection of those agents. O It requires short palindromic regions to function. O It is able to predict which phage will infect a bacterium in the future. O It prevents phage from injecting their nucleic acids into bacteria. O It initiates the production of antibodies specific to the infectious agent.
Use the information gathered in the CRISPR Simulation to answer the following question. The CRISPR/Cas9 system is able to target (and then cleave) invading phage DNA using a randomly generated RNA sequence complementary to part of the phage DNA and bound to Cas9 O machinery that recognizes all foreign DNA O RNA complementary to part of that phage DNA and bound to Cas9 O O a randomly generated DNA sequence complementary to part of the phage DNA and bound to Cas9 DNA complementary to part of that phage DNA and bound to Cas9
Use the information gathered in the CRISPR Simulation to answer the following question. The CRISPRICas9 system has been transferred to mammals and other animals and used to edit genes. This is possible for all of the following reasons except that O CRISPR/Cas9 allows very specific sequence replacement O eukaryotic cells have DNA repair mechanisms of which CRISPR/Cas9 can take advantage O biomolecules such as RNA, DNA, and proteins, work virtually in the same manner in all organisms O CRISPR/Cas9 can be easily inserted into animal cells. O CRISPR/Cas9 is able to override the immune systems of these animals
Use the information gathered in the CRISPR Simulation to answer the following question. If a CRISPR/Cas9-like immune system existed naturally in humans, as well as in bacteria, what would you search for as indications of such a system? clusters of repeated sequences with homology to infectious agents in the o human genome O small nontranslated RNAs with short areas of homology to infectious agents O known viral sequences inserted in the human genome O All of the choices are correct O a protein with homology to Cas9
Use the information gathered in the CRISPR Simulation to answer the following question. Why is it necessary to incorporate the invading viral DNA into the bacterial genome? O This prevents infection from the invading virus. O This causes the viral DNA to be degraded The bacterium can then transcribe the section of the viral gene to create CRISPR/Cas9 complexes O This is the only way the virus can transcribe and translate its DNA for infection O The invading viral DNA is not incorporated into the genome.
Use the information gathered in the CRISPR Simulation to answer the following question. In a bacterium, the guide RNA would originate from O DNA from the bacterial genome O RNA from an invading pathogern O DNA from an invading pathogen O RNA from the bacterial genome O a DNA/RNA hybrid created by researchers
Use the information gathered in the CRISPR Simulation to answer the following question. Once one CRISPRICas9 molecule is made against an invading pathogen, it is active against all invading pathogens. O true O false
Use the information gathered in the CRISPR Simulation to answer the following question. As in the simulation, you attempt to edit a mouse genome to produce a mottled mouse, yet the mouse that you edited still has black fur. Why did this mouse not have the mottled phenotype you engineered? O You have to edit both alleles of the gene; otherwise you will not see the mottled phenotype. Mottled skin color is a dominant trait, so you would have to wait for the offspring of the engineered mouse to see the desired phenotype. One or both of the guide RNAs did not result in a cut in either allele of the Otarget gene. O O The CRISPR/Cas9 system cannot be used successfully in mammals, so you would not see any mottled color mice. The skin color gene was edited in the wrong cell type.
Use the information gathered in the CRISPR Simulation to answer the following question. Which type(s) of mutation can be created using the CRISPR/Cas9 system? O point mutations in the coding region of a gene O deletion of a splicing enhancer of a gene O All of the answer choices are correct. O excision of enhancer sites that lie at a distance down- and upstrearn of a gene O deletion of a single codon in the coding region of a gene
Solution by an expert tutor
Blurred Solution
This question has been solved
Subscribe to see this solution