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Question: please answer the bolded questions...

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tubule walls (called various kinds of tubule cells, as in proximal tubule cells, distal tubule cells, and so forth) are very good for viewing Go to higher power and examine an area containing tubules, the cells should look like the higher power micrograph above. Their most obvious feature, which stains dark purple, is the nucleus; what is staining here is the DNA Would you say that the DNA is probably evenly distributed in the nucleus, or is it more abundant in some areas than in others? If so, where? (Recall that you are looking at slices through the normally spherical nuclei - a cross section.) In many tubule cells you may detect a distinct, small, darker area inside the nucleus -this is the nucleolus. It stains so darkly because it is the site (on one or more chromosomes) where the ribosomal RNA genes are located, and is constantly being transcribed at such a high rate that the RNA (which is also acidic) picks up dyes - as well as a complex protein scaffolding, also acidic, which assembles the ribosomal proteins to the ribosomal RNAs here. The number of nucleoli per cell depends on the species (variations in the number of rRNA genes) and tissue (number of those genes actually active in rRNA synthesis). Can you tell how many nucleoli there are in tubule cells? Can you think of an explanation for cells that seem to lack any nucleoli?

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