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Question: q410 pts please explain quottelomere lengthening q525 pts use the...

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Q4-(10 pts) Please explain Telomere lengthening Q5-(25 pts) Use the diagram of the DNA replication fork below to answer the following questions 5 a) (6 pointsy) Assume the direction of replication is right to left. Draw and label the leading and lagging strands on this diagram. Be sure to label the 5 and 3 ends of both newly synthesized strands b) (3 points) Indicate where helicase would be found and its direction of movement e) (4 points) Indicate the one or more sites where DNA polymerase would be found and its/their direction of d) (8 points) Indicate with a yes/no answer whether the following situations interfere with the replication process movement. diagrammed above Affects leading strand Affects lagging strand i) absence of functional ligase ii) the presence of dideoxy-thymidine: ii) absence of functional telomerase: iv) defective spliceosome machinery:
e) (4 points) The base sequence of a region where DNA replication begins is shown below.What is the sequence sf the primer that is synthesized complementary to the bases in bold? Be sure to indicate the S ands ends S-AGGCCTCGACTTCGTATTGCTTTCAGAA3 06-(24 pts) Please explain only three (3) of the following terms.... &points each Epistasis Helicase, Okazaki fragments, Semiconservative replication, Mutations of haploinsufficient genes are recessive DNA Ligase
01-(25 pts) In sweet peas,the synthesis of purple anthocyanin pigment in the petals is controlled and D. The pathway is gene B gene D white enyme purple enyme, anthocyanin intermediate a) (5 points) What color petals would you expect in a pure-breeding plant unable to catalyze the first reaction? b) (5 points) What color petals would you expect in a pure-breeding plant unable to catalyze the second reaction? c (5 points) If the plants in parts a and b are crossed, what color petals will the F1 plants have? d) (10 points) What ratio of purple : blue : white plants would you expect in the F2? intermediate (blue) Q2- (6 pts) The following table contains statements pertaining to Hfr, F, F. F strains of bacteria, Indicate in the respective boxes if the statements apply to each category by putting amark Each wrong answer will cancel out one correct answer F Statement (A) Contains genes that regulate conjugation and the transfer of DNA from one cell to another (B) Always acts as the recipient in conjugation (C) Cannot form a pilus (D) F factor in the plasmid form carrying a segment of chromosomal DNA Q3-(15 pts) You have isolated two different mutations in the mi gene of phage λ that make tiny plaques. These mutations are called mi-I and mi-2. You cross and mi-1 phage with an mi-2 phage by coinfecting E. coli with phage of both types so that each cell receives at least one phage of each type. Of the 1000 plaques that result from the cross, only 8 form large plaques while the rest are tiny. a) (3 points) Please draw a map showing possible crossovers between two phages. b) (6 points) What is the distance between the mi-1 and mi-2 mutations in map units? c) (6 points) The genome of phage λ is 5x105 bp in physical length and 200 map units in genetic length. what is the distance between the mi-I and mi-2 mutations in base pairs?
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