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Question: question 1 1 pts studies from your lab have shown...

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Question 1 1 pts Studies from your lab have shown that a novel nuclear protein functions as an important transcription factor that activates the transcription of several critical tumor suppressor genes. This transcription factor was found to be downregulated in breast cancer cells. Careful sequence analysis reveals the following sequence in the N-terminus of this protein (the positions of the amino acids are indicated): Pro(1)-Pro(2)-Val (3)-Ala(4)-Arg(5)-Lys(6)-Lys(7)-Arg(8)-Lys(9)-Lys(10)-Lys(11)-Tyr(12) lle(13sP. A construct in which amino acids 1-10 are deleted does not localize to the nucleus. However, when you fuse amino acids 1-10 to GFP, GFP remains in the cytosol Which of the following statements best describes the conclusion that you would draw from this experiment? amino acids 1-10 are necessary but not sufficient to direct the proteins import into the nucleus amino acids 1-10 are necessary and sufficient to target the protein into the nucleus amino acids 1-10 are necessary for nuclear import of this protein because this domain associates with the GEF for the small GTPase Ran amino acids 1-10 are sufficient for the nuclear import of this protein amino acids 1-10 are necessary for nuclear import because they interact with the Ran GTPase Question 2 1 pts In the protein from question 1, Arg in position 8 is mutated to Asp, Lys in position 6 is mutated to an Leu Which of the following statements best describes the phenotype produced by this mutation? ● the nucleus will be misshaped because many proteins, including lamin A, are no longer imported into the nucleus this mutation leads to a general disruption of nuclear import, which ultimately leads to the absence of many transcription factors from the nucleus and an overall defect in gene expression . the mutated protein will remain in the cytosol, where it will now regulate gene expression Ran-GTP will remain in the cytosol because it cannot be converted into the GDP bound state The tumor suppressor genes controlled by this transcription factor will not be transcribed

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