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The Transforming Principle Case Study Bio 449 Name The properties of the rough strain are its phenotype. In the Griffith experiment you read about, the phenotype of the living rough strain changed from nonpathogenic to pathogenic. Scientists proposed that it changed because the genotype changed. The next set of experiments started with the hypothesis that the genotype change was due to some extra genetic material being added to the rough bacteria to change their phenotype to a smooth phenotype Avery, MacLeod, and McCarty had three tools to use in their experiments (in addition to the smooth and rough strains, and mice): 1. Proteases 2. DNase 3. RNase All three of these tools are enzymes, as you can guess because their names end in ase. Questions 1. Given the names of these three enzymes, what reaction do you expect each one to catalyze? Write your answers in the space to the right of the name above 2. If you performed the following experiment what results would you expect if DNA were, in fact, the transforming principle? Explain your conclusion. Heat-killed smooth bacteria RNase Protease DNase Treated with Added to live Rough bacteria Would the mice live or die? 3. Using a well-constructed essay paragraph (on the back of this sheet), describe the experiments by Avery, Macleod, and McCarty that determined the role for DNA as the genetic material (include the logic behind the experiment).

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