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Question: question 1 based on our vector we will need the...

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Question 1 Based on our vector we will need the Xhol and Ndel recognition sequence. Research the sequence for the Xhol cut site: His.Tag Xho Type your response here Ndel pET-28a Promoter

Based on our vector we will need the Xhol and Ndel recognition sequence. Question 2 Research the sequence for the Ndel cut site: His.Tag Xhol Type your response here Ndel pET-28a Promoter

For the following experiment, we want the His.Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein of interest will end up with a C-terminal His.Tag. Question 3 NEW 5 Primer Sequence ype your response here His-Tag Xhol Ndel pET-28a Promoter

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