Question: question 1 using the rate data below answer either true...

QUESTION 1

1. Using the rate data below answer either true or false for the following questions on the effect of various conditions on the enzyme kinetics of papain and BAPNA (in pH 6 buffer unless noted otherwise):

   0.0025M BAPNA Rate=0.00701
   0.0050M BAPNA Rate=0.01404
   0.0050M BAPNA Rate=0.00024 with 0.2M Sodium Citrate
   0.0050M BAPNA Rate=0.02045 at 30 degrees C
   0.0050M BAPNA Rate=0.00402 at 10 degrees C
   0.0050M BAPNA Rate=0.00095 at pH 10
   0.0050M BAPNA Rate=0.00621 at pH 4
   

   -       A.       B.    Increasing substrate concentration (BAPNA) decreases rate of reaction.       -       A.       B.    The reaction is slower in more basic conditions.       -       A.       B.    The reaction is faster at higher temperature.       -       A.       B.    The reaction is faster in more acidic conditions.

   A. True B. False

2 points   

QUESTION 2

1. What is the rate of reaction (units are M/sec) if the slope from the graph is 0.462 (units are 1/sec) for the breakdown of BAPNA to p-nitroanaline by papain? Do Not Include Units

2 points   

QUESTION 3

1. Please anwswer the following True/False questions relating to spectrophotomentry in experiment B:
   

   -       A.       B.    The relationship between concentration and absorbance is always linear even when solutions are very concentrated.       -       A.       B.    Transmittance is linearly porportional to the concentration of analyte or sample in solution.       -       A.       B.    An indicator that has a wavelength for the absorbance maximum of 410 nm will transmit a yellow color.       -       A.       B.    The solution without the analyte (blank) is used to set the absorbance at zero when using a spectrophotometer.       -       A.       B.    If an indicator is yellow it absorbs yellow light.

   A. True B. False

2 points   

QUESTION 4

1. This problem relates to the enzyme kinetics section of this experiment. The first few pages of exp. B in the lab manual will be helpful. Please select either True or False for each of the following statements.

   -       A.       B.    If the rate stays the same when the concentration of BAPNA is doubled the reaction is first order with respect to BAPNA.       -       A.       B.    Rates of 5.0x10-7 and 4.9x10-7 M/sec for 0.0025M and 0.0050M BAPNA respectively suggests zero order.       -       A.       B.    Rates of 1.1x10-7 and 4.5x10-7 M/sec for 0.0025M and 0.0050M BAPNA respectively suggests second order.       -       A.       B.    The rate most likely could not be measured at 630 nm for this experiment.       -       A.       B.    Two concentrations of papain were used to study the rate of reaction in this experiment.

   A. True B. False