1. Engineering
  2. Chemical Engineering
  3. question 1 using the rate data below answer either true...

Question: question 1 using the rate data below answer either true...

Question details

QUESTION 1

  1. Using the rate data below answer either true or false for the following questions on the effect of various conditions on the enzyme kinetics of papain and BAPNA (in pH 6 buffer unless noted otherwise):

    0.0025M BAPNA Rate=0.00701
    0.0050M BAPNA Rate=0.01404
    0.0050M BAPNA Rate=0.00024 with 0.2M Sodium Citrate
    0.0050M BAPNA Rate=0.02045 at 30 degrees C
    0.0050M BAPNA Rate=0.00402 at 10 degrees C
    0.0050M BAPNA Rate=0.00095 at pH 10
    0.0050M BAPNA Rate=0.00621 at pH 4

          -       A.       B.   

    Increasing substrate concentration (BAPNA) decreases rate of reaction.

          -       A.       B.   

    The reaction is slower in more basic conditions.

          -       A.       B.   

    The reaction is faster at higher temperature.

          -       A.       B.   

    The reaction is faster in more acidic conditions.

    A.

    True

    B.

    False

2 points   

QUESTION 2

  1. What is the rate of reaction (units are M/sec) if the slope from the graph is 0.462 (units are 1/sec) for the breakdown of BAPNA to p-nitroanaline by papain? Do Not Include Units

2 points   

QUESTION 3

  1. Please anwswer the following True/False questions relating to spectrophotomentry in experiment B:

          -       A.       B.   

    The relationship between concentration and absorbance is always linear even when solutions are very concentrated.

          -       A.       B.   

    Transmittance is linearly porportional to the concentration of analyte or sample in solution.

          -       A.       B.   

    An indicator that has a wavelength for the absorbance maximum of 410 nm will transmit a yellow color.

          -       A.       B.   

    The solution without the analyte (blank) is used to set the absorbance at zero when using a spectrophotometer.

          -       A.       B.   

    If an indicator is yellow it absorbs yellow light.

    A.

    True

    B.

    False

2 points   

QUESTION 4

  1. This problem relates to the enzyme kinetics section of this experiment. The first few pages of exp. B in the lab manual will be helpful. Please select either True or False for each of the following statements.

          -       A.       B.   

    If the rate stays the same when the concentration of BAPNA is doubled the reaction is first order with respect to BAPNA.

          -       A.       B.   

    Rates of 5.0x10-7 and 4.9x10-7 M/sec for 0.0025M and 0.0050M BAPNA respectively suggests zero order.

          -       A.       B.   

    Rates of 1.1x10-7 and 4.5x10-7 M/sec for 0.0025M and 0.0050M BAPNA respectively suggests second order.

          -       A.       B.   

    The rate most likely could not be measured at 630 nm for this experiment.

          -       A.       B.   

    Two concentrations of papain were used to study the rate of reaction in this experiment.

    A.

    True

    B.

    False

Solution by an expert tutor
Blurred Solution
This question has been solved
Subscribe to see this solution