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Question: question 1 you are a cancer researcher studying skin cancer...

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Question 1.

You are a cancer researcher studying skin cancer cells that have become metastatic (i.e.

they’ve migrated from the site of the original tumor to establish new secondary tumors). You

analyze skin samples from an unaffected patient and from one with the metastatic cancer. After

a series of detergent solublization and centrifugation steps, you separate pellet samples from

each tissue type by gel electrophoresis and find the following results:

Skin normal cancer tissuetissue 250kDa- _A 75kDa- 60kDa-1 __D 30kDa-__F

A. What is the difference between the normal skin sample versus the skin cancer tissue

sample? (2 points)

B. Which protein would you select for further study? (2 points)

C. Many proteins work in complexes with other proteins. How could you determine if the cancer

protein in question above has one or more intracellular binding partners? (6 points)

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