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Question: question 10 2 pt quotconcentration of protein was determined according...

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Question 10 (2 pt) Concentration of protein was determined according to Bradford....Bovine serum albumin was used as external standard..... The absorbance at 595 nm was measured with a UVvisible Spectrometer a) What is the definition of absorbance A ? b) What is the difference between an external standard and an internal standard? c) In the Bradford method a dye -Coomassie Blue G 250 - binds to basic amino acid residues of the protein and after binding the color changes from brown to blue. Over a large protein concentration range a non linear curve is obtained. Why? d) We need to construct a calibration curve with a 1.75 150 125 reference protein. here bovine serum albumin, but sometimes a globulin (BGG) is used; the response is different as shown in the figure Give the approximate protein value in our sample corresponding to A-1 if we are using bovine serum albumin or globulin as the reference protein ? 50 8.00 L500 2.00 2,000 Protein Concentration (ppal)
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