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Question: question 2 ureadenatured hemoglobin a and b chains migrate as...

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QUESTION 2 Urea-denatured hemoglobin a and B chains migrate as bands in an electrophoretic system according to their net charge at a defined pH. The β-chain of Hb Cowtown has a single His->Leu substitution. Given a mixture of normal and Cowtown β chains, grder the chains ascording to their increasing mobility toward the anode (+)relative to each other electrophoresis buffer normal ß-chain Cowtown ß-chain pH 8.9 DH 6 Higher mobility equal mobility The mutation in Hb Cow Lower mobility QUESTION 3 e impairment of the Bohr effect, yet does not alter the equilibrium between the
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