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Question: question 6 1 pts you are expressing gfptagged keratin in...

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Question 6 1 pts You are expressing GFP-tagged keratin in tissue culture cells in order to perform FRAP analysis on keratin bundles. Which of the following graphs (1. 4.) best describes the behavior of keratin after photobleaching with a laser beam, which is done at the Os time point? Images of the subsequent time lapse analysis are shown in the figure on the left (time is shown in seconds Cs 20s 20s 0s 10s 20s 0s 10s 20s 0 10s 20s 0 1 0 2 0 3 Question 7 1 pts The yeast Saccharomyces cerevisae is haploid and can be grouped into two different mating types: a-cells and alpha-cells. Secretion of the mating pheromone a-factor (by an a-cell) and apha-factor (by an alpha-cell) leads to mating, during which a diploid cell is formed (see image) You have isolated an a-cell yeast mutant which grows at normal rate, but is unable to mate with an apha-cell because the mating pheromone a-factor is not secreted. Which of the followine proteins is most likely mutated to produce such a phenotype? O Signal Recognition Particle (SRP) O O O O A subunit of the ribosome the cytosolic chaperone which keeps the protein in the cytosol in an unfolded state the Sec61 protein, a critical component of the translocation channel the signal sequence of a-factor

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