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Remaining Time: 19 minutes, 37 seconds. Question Completion Status àMoving to the next question prevents changes to this answer. Question 7 Why did you expect GFP to come out into the 3rd collection tube? O TE buffer washed the resin column and was collected in the 3rd collection tube. O TE buffer elution released the GFP molecules from the resins. O TE buffer didnt have any ammonium sulfate Before adding TE buffer the resin column was washed with wash buffer TE buffer molecules bind on to the hydrophobic amino acids present in GFP molecules Moving to the next question prevents changes to this answer 名0 4

emaining Time: 17 minutes, 12 seconds Question Completion Status: Δ Moving to the next question prevents changes to this answer. Question8 The GFP protein produced by pGlo transformed E. coli has the following charatetristics D It is translated from 714 nucleotide long mRNA is composed of 238 amino acids. It contains stretches of hydrophobic amino acids. O Its 3D structure appears like a barrel. O It carries hydrophobic amino acids that has strong affinities for molecules of water Δ MOving to the next question prevents changes to this answer. F2 F7 3

Question Completion Status -> Δ Moving to the next question prevents changes to this answer. Question 9 You found that pGlo transformed clls fluoresce. Hence, fluorescence refers to the following Production of green color by a bacteria. O Production of green color by any cell. O Production of red color by any cell. Emitting of high energy light absorbing low energy light. O Emitting of low energy light absorbing high energy light. A Moving to the next question prevents changes to this answer FS 2 4

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