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Question: table 1 pipetting scheme for master mix with coi primer...

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Table 1. Pipetting scheme for master mix with COI primer sets. Add each reagent to the master mix in the order indicated by the number in the parentheses (total per | Number of | . otal μι Needed for Conc. of Stock Solution 5X 10 mM 25 mM 10μΜ 10 uM Final Conc. Master Mix Reactions tube) master mix 1X PCR buffer (1) dNTP mix (3) MgCh (4) Forward Primer (5 Reverse Primer (5 Taq polymerase (6) DNA Water (2 Total volume of the entire reaction (uLs) 200 1.5 mM 0.2 uM 0.2 uM 2 HL 50 μL

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