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  3. table 1 some weak acids and corresponding conjugates commonly used...

Question: table 1 some weak acids and corresponding conjugates commonly used...

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Table 1. Some weak acids (and corresponding conjugates) commonly used to make biochemical buffers. Name/abbreviation Acetic Acid Carbonic Acid pK Bicarbonate pk2 Citric Acid pK Acid Conjugate pKa CH3COOH th CO HCO3 он COOH 4.76 3.77 10.24 HCO3- QH HOOCCH CCH,COOH HOOCCH CCH,Coo 3.13 2C00 HOOCCH2-CCHC001 4.76 OOCCH2CCH COO6.40 COOH Citric Acid pK2 он doo coo H Citric Acid pK; он coo HEPES Ho 7.55 Histidines Imidazole group 6.04 он HN NH2 Succinic Acid pK Succinic Acid pK2 Tris HooCCH CH, Ch, Ch, 005.63 (HOCH2)3CNH
8. A biochemistry student needs to make 500 mL of a 0.025 M citrate buffer with a pH of 5.0 for an enzyme assay. Citric acid (represented as HiA) has three protons that can dissociate, each with different pK, values (Table 1). In lab, the student has to: Na A, NayHA, NaflA, and citrie acid (H,A). Note that when NayA is added to a solution it disassociates to A. access a. Which acid/conjugate pair should the student select to make their buffer? b. Calculate the concentration of the correct acid and conjugate the student will need to make this buffer. (Recall steps from problem 5). c. Suppose in the lab there are the following solutions of NajA, Na2HA, NaH2A, and H.A (citric acid). How many mL of the correct acid and conjugate will be needed to make 500 mL of the 0.025 M citrate buffer (pH 5.0)? 0. 15 M Na3A 0.20 M Na2HA 0.25 M NaH2A 0.30 M HjA (citric acid)

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