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  3. table 113 chromatogram peak areas of sucrose concentrations sucrose concentration...

Question: table 113 chromatogram peak areas of sucrose concentrations sucrose concentration...

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TABLE 1.13 Chromatogram Peak Areas of Sucrose Concentrations Sucrose concentration (g/L) 6.0 12.0 18.0 24.0 30.0 Peak area 55.55, 57.01, 57.95 110.66,114.76, 113.0S 168.90, 169.44, 173.55 233.66, 233.89, 230.67 300.45, 304.56, 301.11 partial pressure of oxygen is 95 mmHg in the artery and 40 mmHg in the vein. The Henrys law constant of oxygen is 0.74 mmHg/uM. Determine the concentration at arterial and venous condition. 1.31 Sucrose concentration in a fermentation broth is measured using HPLC (high-performance liquid chromatography) (Doran, Bioprocess Engineering Principles, 1999). Chromatogram peak areas are measured for five standard sucrose solutions to calibrate the instrument. Measurements are performed in triplicate, with results given in Table 1.13. (a) Determine the mean peak area and standard deviation for each sucrose concentration (b) Plot the mean peak areas as a function of sucrose concentration. (You may use Excel, MATLAB, or another program.) (c) Determine an equation for peak area as a function ofsucrose concentration. (d) A sample containing sucrose gives a peak area of 209.86. What is the sucrose concentration?

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