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  3. the figure to the right is a diagram of the...

Question: the figure to the right is a diagram of the...

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The figure to the right is a diagram of the fingerprint obtained by subjecting a mixture of 18 of the 20 common amino acids (all except Asn and GIn) to electrophoresis in one dimension and chromatography in the other (butanol:acetic . acid:pyridine). The original spot containing all 18 amino acids was at the origin. During electrophoresis, similar sized molecules move primarily due to differences in their net electrical charge, with anions moving toward the anode 5. Brown Yellow positive terminal) and cations moving toward the cathode (negative terminal). During chromatography in this system, molecules move primarily due to differences in polarity, with more OOCO8 Origin polar molecules moving less and less polar molecules moving more. Identify the numbered spots. They correspond to the amino acids Arg, Asp, Cys, Glu, His, lle, Leu, Lys, and Pro. (Hint: There are 9 amino acids, but only 8 spots!) (0.5 pt each) Spot number 1: Spot number 2: Spot number 3 Spot number 4: Spot number 5: Chromatography Spot number 6: Spot number 7: Spot number 8:
3. The pH at which a molecule carries no net electric charge is known as its isoelectric point, pl. For the a-amino acids, where K, and K, are the dissociation constants of the two ionizations involving the neutral species. (p. 86 of your text) Given the values of pKi, pK2, and pKR for the amino acids in the table below, calculate the values of pl for the amino acids Ille, Leu, Pro, Asp, the amino acid will have a net positive amino acid will have a net negative electric charge. Your answers to this question will question 5 below. (0.5 pt each) Glu, Arg, Lys, His, Cys, and Tyr. When the pH of a solution is below the pl value, electric charge; when the pH of a solution is above the pl value, the be useful in answering Amino acid pKi pK2 pKR Ile: Leu: Pro: Asp: Lys: KR+ His: LyVt Cys: Tyr: Arg 1.82 8.99 12.48 Asp 1.99 9.90 3.90 Cys 1,92 10.70 8.37 Glu His 1.80 9.33 6.04 lle Leu 2.33 9.74 Lys Pro 1.95 10.64 Tyr 2.20 9.21 10.46 2.10 9.47 4.0 2.32 9.76 2.16 9.06 10.54
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