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Question: the following are procedures for making known semen swab positive...

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The following are procedures for making known semen swab positive controls. 1) What is the final dilution of the semen? 2) How many swabs will be made? i. Combine 1.5 ml of neat liquid semen with 3.5ml of TE Buffer to prepare a working semen dilution. ii. Inoculate individual swabs with 100ul of the working semen dilution. During the 2hd incubation of a differential extraction, the sperm pellet is incubated in the following lysis buffer. Determine the final concentrations of DTT and prok. ComponentsVolume (uL) Final Conc Digest Buffer 400 12
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